Develop Novel Monoclonal IgG Purification

I need an experienced biochemist to help me create a brand-new, small-scale purification workflow for monoclonal IgG isolated directly from human plasma or serum. The objective is not to follow a routine protocol but to design, test, and document a method that improves yield, purity, or turnaround time over existing approaches. What I bring: • Access to screened human plasma/serum samples and basic downstream analytics (SDS-PAGE, ELISA, UV-Vis). • Laboratory space equipped for column chromatography and standard buffer preparation. What I count on you for: • Critical review of current monoclonal IgG purification literature and identification of process gaps. • Step-by-step experimental design tailored to my lab’s equipment, including detailed buffer compositions and column specifications. • Remote or on-site (if feasible) guidance during two pilot runs, troubleshooting in real time. • Data interpretation and a concise final report comparing the new method against conventional Protein A/G or ion-exchange workflows, highlighting performance metrics and scalability potential. Acceptance criteria 1. ≥90 % purity by SDS-PAGE with consistent band intensity across duplicates. 2. ≥70 % recovery of target IgG from starting material. 3. Clear, reproducible protocol document (reagents, timings, column parameters, QC checkpoints). If you hold a PhD (or equivalent experience) in Biochemistry, Chemistry, Biotechnology, Immunology, or a related discipline and have demonstrated hands-on success purifying IgG from human sources, I would value your expertise on this exploratory project.

Mã dự án: 40238346